연구업적
<연구 소개>
• 기능성 식품 및 의약 소재의 개발 및 생산을 위한 대사공학, 단백질공학, 합성생물학 기술 구축.
• 바이오센서 기반의 고처리능 스크리닝 기술을 활용한 고부가가치 물질 생산용 미생물 세포공장 개발.
• 신규 생리활성 단백질•펩타이드 개발을 위한 무세포 단백질합성 기술 확립.
• 미생물 플랫폼을 기반으로 한 인간형 번역후변형(Post-translational modifications, PTM) 시스템 개발.
<국제 전문학술지>
• SW Baek*, D Ye*, HG Hwang, GY Jung. “Stepwise flux optimization of enhanced GABA production from acetate in Escherichia coli”, Journal of Agricultural and Food Chemistry 72(18), 10420-10427, 2024
• DH Kim*, HG Hwang*, D Ye, GY Jung. “Transcriptional and translational flux optimization at the key regulatory node for enhanced production of naringenin using acetate in engineered Escherichia coli”, Journal of Industrial Microbiology and Biotechnology 51, kuae006, 2024, *co-first authors
• SH Nam*, D Ye*, HG Hwang*, GY Jung. “Convergent synthesis of two heterogeneous fluxes from glucose and acetate for high-yield citramalate production”, Journal of Agricultural and Food Chemistry 72(11), 5797-5804, 2024, *co-first authors (2023 JCR: 92.7%)
• Y Hwang*, HG Hwang*, JY Lee, GY Jung. “Systematic engineering of genistein biosynthetic pathway through genetic regulators and combinatorial enzyme screening”, Journal of Agricultural and Food Chemistry 72(11), 5842-5848, 2024, *co-first authors (2023 JCR: 92.7%)
• HG Hwang*, D Ye*, GY Jung. “Biosensor-guided discovery and engineering of metabolic enzymes”, Biotechnology Advances 69, 108251, 2023 (2022 JCR: 97.8%)
• HG Hwang, J Yang, S Jang, GY Jung. “Riboswitch-guided chalcone synthase engineering and metabolic flux optimization for sustainable production of flavonoids using glycerol”, Metabolic Engineering 75, 143-152, 2023 (2022 JCR: 90.8%)
• DH Kim*, HG Hwang*, GY Jung. “Optimum flux rerouting for efficient production of naringenin from acetate in engineered Escherichia coli”, Biotechnology for Biofuels and Bioproducts 15, 90, 2022, *co-first authors (2021 JCR: 88.36%)
• HG Hwang, MH Noh, Mattheos AG Koffas, S Jang, GY Jung. “Multi-level rebalancing of the naringenin pathway using riboswitch-guided high-throughput screening”, Metabolic Engineering 67, 417-427, 2021 (2020 JCR: 94.3%)
• BE Min*, HG Hwang*, HG Lim, GY Jung. “Optimization of industrial microorganisms: recent advances in synthetic dynamic regulators”, Journal of Industrial Microbiology and Biotechnology 44, 89-98, 2017, *co-first authors
• SC Kim*, BE Min*, HG Hwang, SW Seo, GY Jung. “Pathway optimization by re-design of untranslated regions for L-tyrosine production in Escherichia coli, Scientific Reports 5, 13853, 2015
• HG Hwang*, MS Kim*, SM Shin, CW Hwang. “Risk assessment of the Schmutzdecke of biosand filters: Identification of an opportunistic pathogen in Schmutzdecke developed by unsafe water source”, International Journal of Environmental Research and Public Health 11, 2033-2048, 2014, *co-first authors
<학술회의 발표(selected)>
• HG Hwang, Synthetic Biology-guided metabolic engineering for efficient microbial production of diverse flavonoid compounds, 30th Symposium for Young Asian Biological Engineers’ Community, 2025, Shimane, Japan (International)
• HG Hwang, Programmable post-translational modifications for designer protein synthesis using microbial platforms, International Symposium on Biorefinery and Bioprocess, i-BioJ 2025, Jeju, Korea (International)
•HG Hwang. Synthetic Biology for enhanced biomanufacturing and therapeutic applications, The Korean Scientists and Engineers Community at Cornell (KSECC) Fall Meeting, 2024, Ithaca, USA
•HG Hwang, MH Noh, Mattheos AG Koffas, S Jang, GY Jung. Multi-level optimization of the naringenin biosynthetic pathway, Metabolic Engineering 14 Conference, 2021, On-line (International)
• HG Hwang, S Jang, GY Jung, Systematic rebalancing of flavonoid biosynthetic pathways at transcriptional and translational levels through synthetic RNA biosensors, Korean Society of Industrial and Engineering Chemistry Fall Meeting, 2020, Gwangju, Korea (Excellent Paper Presentation Award)
• HG Hwang, S Jang, GY Jung. Optimization of biosynthetic pathways of Escherichia coli through fine-tuning gene expression based on artificial RNA regulators for enhanced production of flavonoids, 46th Annual Meeting & International Symposium, Korean Society for Microbiology and Biotechnology, 2020, on-line (Excellent Paper Presentation Award)
• HG Hwang, SC Kim, BE Min, SW Seo, GY Jung. Pathway optimization by 5′-UTR redesign for increased production of L-tyrosine in Escherichia coli, i-Bio N 2018: The Symposium on Biorefinery and Bioprocess Topics, Nanjing, China (International, Excellent Paper Presentation Award)
• HG Hwang, S Jang, GY Jung. Evolutionary engineering of Escherichia coli for high production of naringenin using artificial RNA devices, Korean Society of Industrial and Engineering Chemistry Spring Meeting, 2018, Daegu, Korea (Excellent Paper Presentation Award)
< Research Fundings >
• ACS Postdoctoral Fellowships Award Selection, Development of a Cell-Free Human-Type Glycosylation System for the Study and Application of Mucin-Type Glycoproteins in Cancer Research, American Cancer Society, 2025 ($147,500, 2 years)
• Ignite Innovation Acceleration, Research on the Development and bioprocessing of functional glycoproteins, Center for Technology Licensing at Cornell, 2024 ($50,000, 1 year)
< Patents >
• Gyoo Yeol Jung, Yunhee Hwang, Hyun Gyu Hwang. Production of genistein through precise metabolic flux optimization with genetic regulators, Pohang University of Science and Technology, Korea Patent, Application No. 10-2023-0194563
• Gyoo Yeol Jung, Sung Hyun Nam, Dae-yeol Yae, Hyun Gyu Hwang. Improved citramalic acid production yield by establishing a metabolic circuit utilizing glucose and acetic acid simultaneously, Pohang University of Science and Technology, Korea Patent, Application No. 10-2024-0066810
• Matthew DeLisa, Hyun Gyu Hwang, Thomas Crouzier. Processive installation of human-type O-glycans via cell-based and cell-free synthesis, Cornell University, US Patent, Application No. 63/899950
• 기능성 식품 및 의약 소재의 개발 및 생산을 위한 대사공학, 단백질공학, 합성생물학 기술 구축.
• 바이오센서 기반의 고처리능 스크리닝 기술을 활용한 고부가가치 물질 생산용 미생물 세포공장 개발.
• 신규 생리활성 단백질•펩타이드 개발을 위한 무세포 단백질합성 기술 확립.
• 미생물 플랫폼을 기반으로 한 인간형 번역후변형(Post-translational modifications, PTM) 시스템 개발.
<국제 전문학술지>
• SW Baek*, D Ye*, HG Hwang, GY Jung. “Stepwise flux optimization of enhanced GABA production from acetate in Escherichia coli”, Journal of Agricultural and Food Chemistry 72(18), 10420-10427, 2024
• DH Kim*, HG Hwang*, D Ye, GY Jung. “Transcriptional and translational flux optimization at the key regulatory node for enhanced production of naringenin using acetate in engineered Escherichia coli”, Journal of Industrial Microbiology and Biotechnology 51, kuae006, 2024, *co-first authors
• SH Nam*, D Ye*, HG Hwang*, GY Jung. “Convergent synthesis of two heterogeneous fluxes from glucose and acetate for high-yield citramalate production”, Journal of Agricultural and Food Chemistry 72(11), 5797-5804, 2024, *co-first authors (2023 JCR: 92.7%)
• Y Hwang*, HG Hwang*, JY Lee, GY Jung. “Systematic engineering of genistein biosynthetic pathway through genetic regulators and combinatorial enzyme screening”, Journal of Agricultural and Food Chemistry 72(11), 5842-5848, 2024, *co-first authors (2023 JCR: 92.7%)
• HG Hwang*, D Ye*, GY Jung. “Biosensor-guided discovery and engineering of metabolic enzymes”, Biotechnology Advances 69, 108251, 2023 (2022 JCR: 97.8%)
• HG Hwang, J Yang, S Jang, GY Jung. “Riboswitch-guided chalcone synthase engineering and metabolic flux optimization for sustainable production of flavonoids using glycerol”, Metabolic Engineering 75, 143-152, 2023 (2022 JCR: 90.8%)
• DH Kim*, HG Hwang*, GY Jung. “Optimum flux rerouting for efficient production of naringenin from acetate in engineered Escherichia coli”, Biotechnology for Biofuels and Bioproducts 15, 90, 2022, *co-first authors (2021 JCR: 88.36%)
• HG Hwang, MH Noh, Mattheos AG Koffas, S Jang, GY Jung. “Multi-level rebalancing of the naringenin pathway using riboswitch-guided high-throughput screening”, Metabolic Engineering 67, 417-427, 2021 (2020 JCR: 94.3%)
• BE Min*, HG Hwang*, HG Lim, GY Jung. “Optimization of industrial microorganisms: recent advances in synthetic dynamic regulators”, Journal of Industrial Microbiology and Biotechnology 44, 89-98, 2017, *co-first authors
• SC Kim*, BE Min*, HG Hwang, SW Seo, GY Jung. “Pathway optimization by re-design of untranslated regions for L-tyrosine production in Escherichia coli, Scientific Reports 5, 13853, 2015
• HG Hwang*, MS Kim*, SM Shin, CW Hwang. “Risk assessment of the Schmutzdecke of biosand filters: Identification of an opportunistic pathogen in Schmutzdecke developed by unsafe water source”, International Journal of Environmental Research and Public Health 11, 2033-2048, 2014, *co-first authors
<학술회의 발표(selected)>
• HG Hwang, Synthetic Biology-guided metabolic engineering for efficient microbial production of diverse flavonoid compounds, 30th Symposium for Young Asian Biological Engineers’ Community, 2025, Shimane, Japan (International)
• HG Hwang, Programmable post-translational modifications for designer protein synthesis using microbial platforms, International Symposium on Biorefinery and Bioprocess, i-BioJ 2025, Jeju, Korea (International)
•HG Hwang. Synthetic Biology for enhanced biomanufacturing and therapeutic applications, The Korean Scientists and Engineers Community at Cornell (KSECC) Fall Meeting, 2024, Ithaca, USA
•HG Hwang, MH Noh, Mattheos AG Koffas, S Jang, GY Jung. Multi-level optimization of the naringenin biosynthetic pathway, Metabolic Engineering 14 Conference, 2021, On-line (International)
• HG Hwang, S Jang, GY Jung, Systematic rebalancing of flavonoid biosynthetic pathways at transcriptional and translational levels through synthetic RNA biosensors, Korean Society of Industrial and Engineering Chemistry Fall Meeting, 2020, Gwangju, Korea (Excellent Paper Presentation Award)
• HG Hwang, S Jang, GY Jung. Optimization of biosynthetic pathways of Escherichia coli through fine-tuning gene expression based on artificial RNA regulators for enhanced production of flavonoids, 46th Annual Meeting & International Symposium, Korean Society for Microbiology and Biotechnology, 2020, on-line (Excellent Paper Presentation Award)
• HG Hwang, SC Kim, BE Min, SW Seo, GY Jung. Pathway optimization by 5′-UTR redesign for increased production of L-tyrosine in Escherichia coli, i-Bio N 2018: The Symposium on Biorefinery and Bioprocess Topics, Nanjing, China (International, Excellent Paper Presentation Award)
• HG Hwang, S Jang, GY Jung. Evolutionary engineering of Escherichia coli for high production of naringenin using artificial RNA devices, Korean Society of Industrial and Engineering Chemistry Spring Meeting, 2018, Daegu, Korea (Excellent Paper Presentation Award)
< Research Fundings >
• ACS Postdoctoral Fellowships Award Selection, Development of a Cell-Free Human-Type Glycosylation System for the Study and Application of Mucin-Type Glycoproteins in Cancer Research, American Cancer Society, 2025 ($147,500, 2 years)
• Ignite Innovation Acceleration, Research on the Development and bioprocessing of functional glycoproteins, Center for Technology Licensing at Cornell, 2024 ($50,000, 1 year)
< Patents >
• Gyoo Yeol Jung, Yunhee Hwang, Hyun Gyu Hwang. Production of genistein through precise metabolic flux optimization with genetic regulators, Pohang University of Science and Technology, Korea Patent, Application No. 10-2023-0194563
• Gyoo Yeol Jung, Sung Hyun Nam, Dae-yeol Yae, Hyun Gyu Hwang. Improved citramalic acid production yield by establishing a metabolic circuit utilizing glucose and acetic acid simultaneously, Pohang University of Science and Technology, Korea Patent, Application No. 10-2024-0066810
• Matthew DeLisa, Hyun Gyu Hwang, Thomas Crouzier. Processive installation of human-type O-glycans via cell-based and cell-free synthesis, Cornell University, US Patent, Application No. 63/899950